Journal: Cerebrospinal Fluid Research

Article Title: Co-localization and regulation of basic fibroblast growth factor and arginine vasopressin in neuroendocrine cells of the rat and human brain

doi: 10.1186/1743-8454-7-13

Figure Lengend Snippet: Altered distribution of FGF2 in the rat neurohypophysis after dehydration: In normal (A & B) and water-deprived rats (C & D), tissues were immunostained with polyclonal antibodies against FGF2: To visualize the changes in the localization of FGF2 in the extracellular matrix, tissue sections were treated with antibody Ab773 (A & C). To visualize the changes in intracellular FGF2, tissue sections were treated with antibody Ab106 (B & D). In control animals, FGF2 is associated with basement membranes (arrowheads), Herring bodies of axons of neurosecretory neurons (stars) (A) and pituicytes (arrows). Notice the characteristic beaded appearance of axons (B). In neurohypophyseal tissue from experimental rats, (C) and (D) show that the characteristic morphological changes associated with chronic dehydration are accompanied by increased FGF2 staining in structures underlying the perivascular space (arrowheads) (C). The hypertrophic pituicytes (arrows) also display strong nuclear staining (D). (Magnification bar = 100 μm)

Article Snippet: Immunohistochemical procedures used specific polyclonal antibodies raised against human FGF2 (Ab773) and AVP (Immunostar, Hudson, WI, USA).

Techniques: Control, Staining

Journal: Cerebrospinal Fluid Research

Article Title: Co-localization and regulation of basic fibroblast growth factor and arginine vasopressin in neuroendocrine cells of the rat and human brain

doi: 10.1186/1743-8454-7-13

Figure Lengend Snippet: Altered distribution of FGF2 in choroid plexus of rat brain after dehydration: FGF2 immunostaining in lateral ventricle choroid plexus tissues was evaluated in adult rats exposed to chronic water deprivation for 72 h. Anatomically, the choroidal villus consists of a single layer of cuboidal epithelial cells that circumferentially surrounds an inner vascular core and extracellular matrix. The apical membrane faces the 'outside' CSF compartment; see Smith et al. for a delineated description of choroid structural features. Note the marked FGF2 immunostaining in plexus parenchymal epithelium from a dehydrated animal (A), particularly in the nuclei, cytoplasm and the apical (CSF-facing) membrane, and the lower concentration in basement membranes (B). Comparatively, normal animals show lower levels of intracellular FGF2 in the epithelium (C), but stronger staining associated with basement membranes (D). Tissues were immunostained with polyclonal antibodies against FGF2: Ab106 (A & C) and Ab773 (B & D).

Article Snippet: Immunohistochemical procedures used specific polyclonal antibodies raised against human FGF2 (Ab773) and AVP (Immunostar, Hudson, WI, USA).

Techniques: Immunostaining, Membrane, Concentration Assay, Staining

Journal: Cerebrospinal Fluid Research

Article Title: Co-localization and regulation of basic fibroblast growth factor and arginine vasopressin in neuroendocrine cells of the rat and human brain

doi: 10.1186/1743-8454-7-13

Figure Lengend Snippet: Immunoblotting confirms enhanced hypothalamic FGF2 expression after dehydration: FGF2 protein level was analyzed by western blotting for control (C) and dehydrated (D) states. Note the increased FGF2 levels in hypothalamus, resulting from 3 days of water deprivation, for the 3 isoforms of about 18, 23 and 24 kDa. Heart, kidney and brain rat tissues were extracted as described in the text and examined for rat FGF2 with a polyclonal antibody recognizing 3 forms of endogenous FGF2. Equal amounts of protein were loaded per well. Exposure time was 36 h. Dehydration specifically elevated the expression of FGF2 in hypothalamus but not in heart or kidney.

Article Snippet: Immunohistochemical procedures used specific polyclonal antibodies raised against human FGF2 (Ab773) and AVP (Immunostar, Hudson, WI, USA).

Techniques: Western Blot, Expressing, Control